| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137671 |
| Chromosome: | chromosome 6 |
| Location: | 2809075 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271300 | HFO8 | Histone H4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAAGACACCCAGCGAATCCCGGGCTATG |
| Internal bar code: | TTGTAGGATTGGGCCCCCCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1213 |
| LEAP-Seq percent confirming: | 97.6526 |
| LEAP-Seq n confirming: | 1248 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTTGGTGCTAGAGGAGC |
| Suggested primer 2: | CAGACTGGCCAACAACTGAA |