Insertion junction: LMJ.RY0402.137678_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.137678
Chromosome:chromosome 17
Location:3190190
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g722000 MFT30,SOC1 Major facilitator superfamily transporter; (1 of 1) K08214 - MFS transporter, OCT family, solute carrier family 22 (organic cation transporter), member 18 (SLC22A18) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGACCATGTACTGCCTCCCTGTTACATG
Internal bar code:CCCTCGTCGTACGTGGACAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:895
LEAP-Seq percent confirming:98.4686
LEAP-Seq n confirming:7973
LEAP-Seq n nonconfirming:124
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGCCTCTTTAAAACGGAA
Suggested primer 2:GCTTCATTGAGTCGGGGATA