| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137684 |
| Chromosome: | chromosome_2 |
| Location: | 7346058 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g145100 | FAP39 | P-type ATPase/cation transporter, flagellar associated | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAAACAAGATGTGCTCAGTCTTCAGCCCTT |
| Internal bar code: | GTGTCGGAATTGGGCCCCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 970 |
| LEAP-Seq percent confirming: | 98.8526 |
| LEAP-Seq n confirming: | 17317 |
| LEAP-Seq n nonconfirming: | 201 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTCGTTCCTGTGTATGT |
| Suggested primer 2: | CTGTGGGTGAGGTTGGTCTT |