| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.137691 |
| Chromosome: | chromosome 4 |
| Location: | 1192384 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215500 | (1 of 1) PF09735 - Membrane-associated apoptosis protein (Nckap1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATCGCCCAGCTGCTGACGGAGCTACAC |
| Internal bar code: | GCATTGTTTTTCGATAACCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 96.3824 |
| LEAP-Seq n confirming: | 10577 |
| LEAP-Seq n nonconfirming: | 397 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTTTAGTTTGGGCTGGTA |
| Suggested primer 2: | ATCAGGACGTGGTGTTACCC |