Insertion junction: LMJ.RY0402.137693_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.137693
Chromosome:chromosome 6
Location:1796025
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g262400 (1 of 1) K14676 - lysophospholipid hydrolase (NTE, NRE) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACCTCTTATGACAGCGGCAGCGGCGGC
Internal bar code:CAATCGGCCCCACCACCTCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:521
LEAP-Seq percent confirming:99.5244
LEAP-Seq n confirming:2302
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGTCTAGACCACCACCAC
Suggested primer 2:CAGGGAGTGAGCTGAGGAGA