| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137703 |
| Chromosome: | chromosome 1 |
| Location: | 3078021 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019051 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTTAACACACAGCCCACACGCACACAC |
| Internal bar code: | CTATGCGTCAGGCCGAAGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 828 |
| LEAP-Seq percent confirming: | 99.7592 |
| LEAP-Seq n confirming: | 7457 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTAAGCAGGGACTTGTGT |
| Suggested primer 2: | GGACTGGATGGACTTCCTGA |