Insertion junction: LMJ.RY0402.137714_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.137714
Chromosome:chromosome 2
Location:2847841
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g094700 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACACGCGCTTTGATGAGGGCCTTTGTTGT
Internal bar code:TGTGGGAGGCAGGTTATTCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:858
LEAP-Seq percent confirming:99.5681
LEAP-Seq n confirming:2075
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTTCGCTCTCTCTTAAA
Suggested primer 2:GCATACCTTGCGGTTTTTGT