Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.137766 |
Chromosome: | chromosome_8 |
Location: | 987145 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g362000 | FAP72 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGTTCCGAGGCGCACAGGTGCTCGCCAAGG |
Internal bar code: | CCTGTTACTACGGCCGTGCTAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 804 |
LEAP-Seq percent confirming: | 97.181 |
LEAP-Seq n confirming: | 4654 |
LEAP-Seq n nonconfirming: | 135 |
LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTCAGAAACTGCCTTGTCG |
Suggested primer 2: | GAAGGAGGACGGTACACCAA |