Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.137774 |
Chromosome: | chromosome_8 |
Location: | 1913756 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g367250 | FAP176 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTGACGAAGAGGGTTTGGGAAGAAGGGGG |
Internal bar code: | GAGCGTCCGACCTTTTGCGTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1044 |
LEAP-Seq percent confirming: | 99.5795 |
LEAP-Seq n confirming: | 4736 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCATAGACCTGTTGCTGGG |
Suggested primer 2: | ACCATGCATATGCCAGTTCA |