| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137778 |
| Chromosome: | chromosome_1 |
| Location: | 1015788 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g005813 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGTCAGGACCGCAAGCGAACCGGGGGAGG |
| Internal bar code: | CCGCGAGTGTAGGGGTTAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1260 |
| LEAP-Seq percent confirming: | 99.0589 |
| LEAP-Seq n confirming: | 4000 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCTTGGCTGTCGCCTTAT |
| Suggested primer 2: | AGTCGGAGCCCATGTAACAC |