| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137782 |
| Chromosome: | chromosome 14 |
| Location: | 2494322 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625150 | PGM8 | (1 of 1) PTHR23029:SF26 - PROTEIN Y18H1A.4-RELATED; Phosphoglycerate mutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCGCGTGCAAGTTTCCCTGCTGTTCTC |
| Internal bar code: | AGGCGCAATGGTTTACTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2908 |
| LEAP-Seq percent confirming: | 92.4171 |
| LEAP-Seq n confirming: | 40414 |
| LEAP-Seq n nonconfirming: | 3316 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTTTGCATTGGGTTCGTT |
| Suggested primer 2: | ATTGGGAATAAGGGGTCGAG |