Insertion junction: LMJ.RY0402.137800_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.137800
Chromosome:chromosome 4
Location:3758102
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g229494 (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAAGCAACTCGCCAAAGCCACCTGTCGGA
Internal bar code:TTGGTTCGCATAGTTTAACAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:605
LEAP-Seq percent confirming:98.9323
LEAP-Seq n confirming:18068
LEAP-Seq n nonconfirming:195
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAAGGTCCCTCTCCCTCT
Suggested primer 2:CAGACTCTCAAGGACTGCCC