| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137801 |
| Chromosome: | chromosome 7 |
| Location: | 2953192 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332901 | (1 of 80) IPR003882 - Pistil-specific extensin-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCAACGGCGGCAGCGCAGGTGCGGCGG |
| Internal bar code: | CCACACATGATAGCCTCTTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 660 |
| LEAP-Seq percent confirming: | 98.6104 |
| LEAP-Seq n confirming: | 32571 |
| LEAP-Seq n nonconfirming: | 459 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCTACGGATGTCAGTCA |
| Suggested primer 2: | AGAAATGCTTGGGGTGTTTG |