| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137806 |
| Chromosome: | chromosome 7 |
| Location: | 4892322 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACACGGCCAGTAGCCTGATGGGTTAGT |
| Internal bar code: | AAGTATCCGCGATTGAACTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 98.6891 |
| LEAP-Seq n confirming: | 7905 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCCTGCACAACCCATTT |
| Suggested primer 2: | GCTTCTTGTTTGTCATGCGA |