| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137813 |
| Chromosome: | chromosome 1 |
| Location: | 3653431 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023550 | FAP234,TPG2 | Flagellar Associated Protein 234; (1 of 1) PTHR24365//PTHR24365:SF301 - TOLL-LIKE RECEPTOR // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGCGTGAACTGCGCTAGGATTGCTTCCG |
| Internal bar code: | AGGAGATCGCGCTTCTGTCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 105 |
| LEAP-Seq percent confirming: | 94.0928 |
| LEAP-Seq n confirming: | 446 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAATTTGAGCACCTGGTTT |
| Suggested primer 2: | CCATTCTGGACAGCCTTGAT |