| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.137823 |
| Chromosome: | chromosome 1 |
| Location: | 2668227 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015600 | (1 of 1) PTHR23346//PTHR23346:SF7 - TRANSLATIONAL ACTIVATOR GCN1-RELATED // TRANSLATIONAL ACTIVATOR GCN1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCGACGGCCTGGGTGACCTGGTGTCG |
| Internal bar code: | CTAAGCCGCGGATCGCCTGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 131 |
| LEAP-Seq percent confirming: | 99.8004 |
| LEAP-Seq n confirming: | 500 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCGCCACAAAACAACAA |
| Suggested primer 2: | ACTCCCACCCCTTCAGTTCT |