| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.137847 |
| Chromosome: | chromosome 12 |
| Location: | 5958743 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534600 | TOM20 | (1 of 1) PTHR32409:SF3 - MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20-1-RELATED; Mitochondrial translocase of outer membrane, 20 kDa | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGTGCGTTGCATTGCTAAGATCTCATA |
| Internal bar code: | CTCCGTTGTGAGCACGGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 99.5424 |
| LEAP-Seq n confirming: | 4786 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGCCTCTTCTTTCAGTG |
| Suggested primer 2: | CGTCGGTGTTGTTACGAATG |