| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137848 |
| Chromosome: | scaffold 24 |
| Location: | 21287 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755247 | (1 of 1) IPR003010//IPR020683 - Carbon-nitrogen hydrolase // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTATCACATCTTCCACGACCCCAACTCGT |
| Internal bar code: | CTTCAATGTGAGTCTGAATAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 17.7966 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 97 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCACTTAGCGGAAGCGTT |
| Suggested primer 2: | ATATCCCTCCAGACCAACCC |