Insertion junction: LMJ.RY0402.137871_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.137871
Chromosome:chromosome 9
Location:2432802
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g391500 ATG9 (1 of 1) K17907 - autophagy-related protein 9 (ATG9); Autophagy related 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCACGTGTGCGTACGGTACATGACGGA
Internal bar code:GGCACGCATAAATTGTCGCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:599
LEAP-Seq percent confirming:99.9554
LEAP-Seq n confirming:4478
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GTACGCTCCCAGTGCTCTTC
Suggested primer 2:TCACATAGGCGCAAACTCAG