| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137908 |
| Chromosome: | chromosome 17 |
| Location: | 3386442 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723900 | FAP131,ROC103 | Flagellar Associated Protein 131 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATAAGAATAGATGCTGCTATACCAGAA |
| Internal bar code: | CCGCCTGATCAAGATGGGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1247 |
| LEAP-Seq percent confirming: | 99.5739 |
| LEAP-Seq n confirming: | 12620 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCAGGCACGCTTAGATA |
| Suggested primer 2: | GTAGGGTCGTGCTTGTTGGT |