| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137912 |
| Chromosome: | chromosome 3 |
| Location: | 4591716 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176961 | (1 of 37) IPR009030 - Insulin-like growth factor binding protein, N-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGTATCTACATACCGTCTACCACTTCC |
| Internal bar code: | ATGCGCGATCGTACCACTAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 86.1538 |
| LEAP-Seq n confirming: | 616 |
| LEAP-Seq n nonconfirming: | 99 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGAAGCAGAGGGACCAGT |
| Suggested primer 2: | ACGTGTCATATCAGCACCCA |