Insertion junction: LMJ.RY0402.137921_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.137921
Chromosome:chromosome 11
Location:3399411
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g480700 (1 of 2) IPR000104//IPR000945 - Antifreeze protein, type I // Dopamine beta-hydroxylase-related intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAATGCTGTTGCCTTTTCGCTCGCGCGCGT
Internal bar code:AGTGGTCTTGAAAACGAAGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:324
LEAP-Seq percent confirming:99.184
LEAP-Seq n confirming:6199
LEAP-Seq n nonconfirming:51
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCCCAAACTAAACCAAA
Suggested primer 2:GTACAGCATGATCACGTCGC