Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.137921 |
Chromosome: | chromosome 11 |
Location: | 3399411 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g480700 | (1 of 2) IPR000104//IPR000945 - Antifreeze protein, type I // Dopamine beta-hydroxylase-related | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGCTGTTGCCTTTTCGCTCGCGCGCGT |
Internal bar code: | AGTGGTCTTGAAAACGAAGGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 324 |
LEAP-Seq percent confirming: | 99.184 |
LEAP-Seq n confirming: | 6199 |
LEAP-Seq n nonconfirming: | 51 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
Suggested primer 2: | GTACAGCATGATCACGTCGC |