| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137936 |
| Chromosome: | chromosome 10 |
| Location: | 173932 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418950 | KIN10-2,KIN10B,KIN4-4,IAR1 | Kinesin motor protein; (1 of 2) PTHR24115:SF136 - KLP31E, ISOFORM A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGCGTATCCTACCTAATCGAGAGCTGT |
| Internal bar code: | TCTCTAGGCCCCGGTGCTCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 98.9302 |
| LEAP-Seq n confirming: | 4069 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGCCATTTGCCTAGATT |
| Suggested primer 2: | CTCTATGCGCTATGCAACCA |