| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137939 |
| Chromosome: | chromosome_2 |
| Location: | 1053489 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g080700 | BIP1 | Endoplasmic reticulum associated Hsp70 protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGCACATGTGTGCCACCCCTGCTCTCCTC |
| Internal bar code: | ACGGATTAAGGGTTAGGTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1031 |
| LEAP-Seq percent confirming: | 98.6124 |
| LEAP-Seq n confirming: | 2203 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGGGGAGATACTGGGAA |
| Suggested primer 2: | GAGCCCTAGAGGCTTGTGTG |