Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.137978 |
Chromosome: | chromosome_3 |
Location: | 5648477 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g187400 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGCCGCCGCCCCTGCCGCCCGTGCGGCCGT |
Internal bar code: | ATTAATGAGCTCTTCCGGCTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 199 |
LEAP-Seq percent confirming: | 96.6833 |
LEAP-Seq n confirming: | 583 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCGCACTTTACCCAGAAGC |
Suggested primer 2: | CCAGGTGCAGGTCCGTAT |