| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137981 |
| Chromosome: | chromosome 9 |
| Location: | 3845545 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391875 | (1 of 1) PTHR22847//PTHR22847:SF453 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCCTCGAGATCGGGCACTTCCACGCAC |
| Internal bar code: | TGATTCAGGATTGGCTAGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1239 |
| LEAP-Seq percent confirming: | 99.2091 |
| LEAP-Seq n confirming: | 2007 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGACAGTGGTAAGGCAG |
| Suggested primer 2: | TTTGGCGAGATAGCTGGAGT |