| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137992 |
| Chromosome: | chromosome 10 |
| Location: | 6008601 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462850 | (1 of 2) PTHR10283//PTHR10283:SF21 - SOLUTE CARRIER FAMILY 13 MEMBER // ARSENICAL PUMP MEMBRANE PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCAGGTGGGACTGATGGTGTTTATATT |
| Internal bar code: | TTCGCTGAGCTCAATGCTCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 468 |
| LEAP-Seq percent confirming: | 97.4026 |
| LEAP-Seq n confirming: | 150 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTTAATCACTACGCCTCC |
| Suggested primer 2: | TAGCTGCTGTTGTGGTGAGG |