| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137997 |
| Chromosome: | chromosome 3 |
| Location: | 4251251 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174200 | (1 of 9) IPR000210//IPR011042//IPR011333 - BTB/POZ domain // Six-bladed beta-propeller, TolB-like // POZ domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAACGGCAGCGCTGGCGATCTGAGGATTT |
| Internal bar code: | GCAAGCCCGGGCAGTGCTAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 902 |
| LEAP-Seq percent confirming: | 97.5514 |
| LEAP-Seq n confirming: | 3466 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCGGGGCTGTGTATCAT |
| Suggested primer 2: | TAGCACCCTTTGCCCATATC |