| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.138010 |
| Chromosome: | chromosome 4 |
| Location: | 2754194 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223650 | FAP30 | (1 of 1) PF02494 - HYR domain (HYR); Flagellar Associated Protein 30 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCATTGGCGGGCGGGCCCAACAATGCA |
| Internal bar code: | TCTCTCCAGCTCTCCGGAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 91.8684 |
| LEAP-Seq n confirming: | 9829 |
| LEAP-Seq n nonconfirming: | 870 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCCATTCTTCATTTGCT |
| Suggested primer 2: | GGCAGTCGTGGTTGGATAGT |