| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138052 |
| Chromosome: | chromosome 12 |
| Location: | 5352280 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529550 | CALK1,CALK,ALK1 | Aurora-like kinase; (1 of 1) PF00069//PF07714//PF14531 - Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) // Kinase-like (Kinase-like) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGATCTTAGCTGACAACACAGGAAGCGGA |
| Internal bar code: | TTCCGTTGGGCGCCCAGAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 883 |
| LEAP-Seq percent confirming: | 99.7356 |
| LEAP-Seq n confirming: | 8676 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGCCTTGACAAGAAAGG |
| Suggested primer 2: | GGTACCAGAGCTGGCTTGAG |