| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138057 |
| Chromosome: | chromosome 1 |
| Location: | 3619501 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023350 | THO2 | Oligoendopeptidase; (1 of 1) 3.4.24.16 - Neurolysin / Neurotensin endopeptidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAATTTGGGCCAGCAGTTGACTGCCCCCG |
| Internal bar code: | TTGGCGGCGGGTCCTAAGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 609 |
| LEAP-Seq percent confirming: | 98.7126 |
| LEAP-Seq n confirming: | 3757 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGACGAGGGAGAAGAGG |
| Suggested primer 2: | AAGACATCCGTAAACCGTCG |