| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.138060 |
| Chromosome: | chromosome 1 |
| Location: | 3431588 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021900 | PUS11 | (1 of 1) PTHR11142:SF10 - TRNA PSEUDOURIDINE SYNTHASE; RNA pseudouridine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCCCCAGGTGCCCGCGTGTGCAGCAGC |
| Internal bar code: | CGGGCCCACGCGTCGCCGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 906 |
| LEAP-Seq percent confirming: | 99.4101 |
| LEAP-Seq n confirming: | 8089 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGCAAAGAAGGAAACAC |
| Suggested primer 2: | AACAAGACAACTGTTCGGGG |