Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.138067 |
Chromosome: | chromosome 9 |
Location: | 3728002 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGAATGCACCCGTCTGTGCCCCCACGAC |
Internal bar code: | GGACCGGGACGCTCGCGACTAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 934 |
LEAP-Seq percent confirming: | 93.0723 |
LEAP-Seq n confirming: | 7510 |
LEAP-Seq n nonconfirming: | 559 |
LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCAACACATCTGCTCAACA |
Suggested primer 2: | GAAAATGGACATGCGGCTAT |