| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.138070 |
| Chromosome: | chromosome 16 |
| Location: | 4854521 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686050 | (1 of 1) PF05277 - Protein of unknown function (DUF726) (DUF726) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGATCCTTCTCACCCCCAACAACAACA |
| Internal bar code: | TGGCCCGCTAGGGAGCTTTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 605 |
| LEAP-Seq percent confirming: | 95.9635 |
| LEAP-Seq n confirming: | 737 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACATCTATACACACGCC |
| Suggested primer 2: | GAGAGGCGGGTATTGTGTGT |