| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138076 |
| Chromosome: | chromosome 12 |
| Location: | 7435779 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCATTCCGCGGGACCGCCGCCGCACAAA |
| Internal bar code: | GCCTTTGGGTCAAGCGATGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 357 |
| LEAP-Seq percent confirming: | 91.4459 |
| LEAP-Seq n confirming: | 35898 |
| LEAP-Seq n nonconfirming: | 3358 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCATATCCACCTCCACC |
| Suggested primer 2: | GGCTGCACAAGCACAAGATA |