| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138080 |
| Chromosome: | chromosome 3 |
| Location: | 4193406 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174000 | EFG11,LPA2 | Mitochondrial translation elongation factor EFG/EF2, LepA-related; (1 of 1) PTHR23115:SF97 - TRANSLATION FACTOR GUF1, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGGAGCAGGACAAGCCCCAAACACTGC |
| Internal bar code: | GTCGTGTTCCGGACCCTAGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 251 |
| LEAP-Seq percent confirming: | 6.11017 |
| LEAP-Seq n confirming: | 792 |
| LEAP-Seq n nonconfirming: | 12170 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCATAACAACCTTGGCCC |
| Suggested primer 2: | AAGATGAAGGACCTGCTGGA |