| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138088 |
| Chromosome: | chromosome 5 |
| Location: | 380263 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233051 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGGTGAGCAAATGAACCCACACGATAGC |
| Internal bar code: | GGCGGACATCAAAGGGGACCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 145 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTGTAAATACCATTGCG |
| Suggested primer 2: | TCGCAGTTAGGCAGGAATCT |