| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138098 |
| Chromosome: | chromosome 12 |
| Location: | 4761134 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524000 | SUC1,GTR7 | (1 of 1) 2.4.1.14 - Sucrose-phosphate synthase / UDP-glucose-fructose-phosphate glucosyltransferase; Sucrose-phosphate synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCACCCAATCATACGCGCACGCACAAAC |
| Internal bar code: | TGTACAAAGAGCAGATTTGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 25 |
| LEAP-Seq percent confirming: | 99.6785 |
| LEAP-Seq n confirming: | 1240 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAAGGGAGGAGCGAGGAG |
| Suggested primer 2: | CTCCCCATTCAGTCCCTACA |