Insertion junction: LMJ.RY0402.138101_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.138101
Chromosome:chromosome 6
Location:7806543
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g302600 (1 of 2) IPR002014//IPR008942 - VHS domain // ENTH/VHS 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTTCCAGTCCGATGATGTGCATTTCGGC
Internal bar code:GGTGGCGCATCGCTGGGAACAG

Confirmation - LEAP-Seq

LEAP-Seq distance:232
LEAP-Seq percent confirming:95.8455
LEAP-Seq n confirming:10843
LEAP-Seq n nonconfirming:470
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGGCCAGGTCGTACTCAG
Suggested primer 2:GTCGAAACACTATGCGCTGA