| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138103 |
| Chromosome: | chromosome 3 |
| Location: | 4076973 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172750 | (1 of 1) IPR002893//IPR012677 - Zinc finger, MYND-type // Nucleotide-binding alpha-beta plait domain | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGGCAGTGCCCCGCCGTGTGTGCTCTA |
| Internal bar code: | TACAGGTGCACCCGTACTGTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 99.7142 |
| LEAP-Seq n confirming: | 6630 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCGCCATGTCAAGTGAAG |
| Suggested primer 2: | GACTGCTTGACTTGATGGCA |