| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138121 |
| Chromosome: | chromosome 2 |
| Location: | 9223100 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGGGCAATGGAAGTCCCACCCCGTACC |
| Internal bar code: | TCTGACTGCCTCGTGGTAGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 93.7076 |
| LEAP-Seq n confirming: | 6359 |
| LEAP-Seq n nonconfirming: | 427 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAACCCTAAAACCCTAAAACCC |
| Suggested primer 2: | GTAGGCGCAGGATGTAGAGC |