Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.138124 |
Chromosome: | chromosome_12 |
Location: | 6475229 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g538300 | LF5,FAP247 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCCACCAGGCTGCTGTGCCATCCGAGTGCC |
Internal bar code: | GGGATGCGTCCAGGTATTTTAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 851 |
LEAP-Seq percent confirming: | 98.6979 |
LEAP-Seq n confirming: | 3032 |
LEAP-Seq n nonconfirming: | 40 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCTCCGCGAGGTTAAGATG |
Suggested primer 2: | ACTCACCCAATAGCCCACAG |