Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.138126 |
Chromosome: | chromosome_9 |
Location: | 4494091 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g395769 | FAP177 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGCTGACACACCCCGCCACCCTCTGCTCT |
Internal bar code: | GCGGCTCGCAGCTCCAGCCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 307 |
LEAP-Seq percent confirming: | 88.0358 |
LEAP-Seq n confirming: | 13289 |
LEAP-Seq n nonconfirming: | 1806 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAAGGGAAAACATGCAAACG |
Suggested primer 2: | ATCGCTGAACCCTACCACTG |