| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138129 |
| Chromosome: | chromosome 11 |
| Location: | 2548821 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476100 | OPR50,MCD1 | petD mRNA stabilization factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGATTGTGTGGTGGTGCTAGGTTGTTG |
| Internal bar code: | CAAGGTCCGTCCACTAGGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 331 |
| LEAP-Seq percent confirming: | 99.3573 |
| LEAP-Seq n confirming: | 3401 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTTACGTTGCACTGGT |
| Suggested primer 2: | GTTTGACGACCACGTCTGG |