| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138136 |
| Chromosome: | chromosome 16 |
| Location: | 2375943 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g660000 | CMT1A,CPLD63 | (1 of 2) PTHR12608:SF7 - GDT1-LIKE PROTEIN 2, CHLOROPLASTIC; Mn transport into chloroplast | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTTGCTAGCGCGCATACACCACAGCAA |
| Internal bar code: | AGGGGCGCTATGGATGCCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 962 |
| LEAP-Seq percent confirming: | 99.6485 |
| LEAP-Seq n confirming: | 13326 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGGTGTTGTAGATCTGGT |
| Suggested primer 2: | AATAAGCTGGAGCTGTGCGT |