| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138174 |
| Chromosome: | chromosome 3 |
| Location: | 7999084 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201700 | (1 of 1) PF15072 - Domain of unknown function (DUF4539) (DUF4539) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTCAGCCCGCCACACACAGCGCAACGA |
| Internal bar code: | CGCGTTGTAGCGCGCGCGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 665 |
| LEAP-Seq percent confirming: | 99.8626 |
| LEAP-Seq n confirming: | 1454 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAAAGCACTGCCTATGTT |
| Suggested primer 2: | CAGCAACAGCTCCAAGTGAG |