| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138188 |
| Chromosome: | chromosome 16 |
| Location: | 851901 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647800 | (1 of 1) IPR000104//IPR001932//IPR015655 - Antifreeze protein, type I // PPM-type phosphatase domain // Protein phosphatase 2C | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCACACGCCGTCGCAGGCGCAGATGAGG |
| Internal bar code: | TTTCATTCATCTTAACCTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 218 |
| LEAP-Seq percent confirming: | 98.4356 |
| LEAP-Seq n confirming: | 818 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCGCTTTCACTGTGATG |
| Suggested primer 2: | CATCAGCTCATGAACTCCGA |