| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138214 |
| Chromosome: | chromosome 17 |
| Location: | 3241910 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722650 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGGTAAGCGGTAGCGGAGGCCTTAGCC |
| Internal bar code: | GAAGCGCGCAGGTAGTGGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 99.7125 |
| LEAP-Seq n confirming: | 28791 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATATGTGCAGGCCCGTCT |
| Suggested primer 2: | TCCACTCCTTCACCTTCACC |