| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138222 |
| Chromosome: | chromosome_7 |
| Location: | 624692 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g316992 | antisense | CDS|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGGCTGGGCTCAGCAGTGGCGTCGGCGCC |
| Internal bar code: | TCATTTTATCCCATCGTATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 205 |
| LEAP-Seq percent confirming: | 99.736 |
| LEAP-Seq n confirming: | 4533 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGAGCAGTCGGTCGTAG |
| Suggested primer 2: | GAGAGCGAGGATGGATTCAG |