Insertion junction: LMJ.RY0402.138229_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.138229
Chromosome:chromosome 2
Location:3841145
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g095750 ZEP1,ZXE1 (1 of 1) 1.14.13.90 - Zeaxanthin epoxidase / Zea-epoxidase; Putative zeaxanthin epoxidase-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCATGGGGAGGGTGGGCCCAAAGTCTG
Internal bar code:GCAACGGATTGACAGTGAGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:346
LEAP-Seq percent confirming:20.6342
LEAP-Seq n confirming:1835
LEAP-Seq n nonconfirming:7058
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GGGATTAGTGTCCACACGCT
Suggested primer 2:TGAACCCCTCTCTCCACAAC